Splitless Solvent Effect
Solvent focusing involves injection of the sample solvent at an oven temperature at least 10- 20° C below the boiling point of the solvent. In this way, the solvent completely condenses on the front of the capillary column. For splitless injection, for a splitless time of 1 minute and one mL liner volume, the initial peak width will be approximately one minute wide. Solvent focusing occurs by evaporation first of the solvent at the injector end of the column and the analytes become more and more concentrated in the ever decreasing solvent band as the oven heats up. The analytes will have a greater affinity for the solvent than for the gas phase. Eventually all solvent will evaporate and at this stage, the analytes will have been focused as a narrow band on the column.
|<< Splitless Injection Purge Time||