- Must be high enough to ensure a narrow sample band. If the split flow is too low (eg. 10mL/ min) the peaks will become very broad because it takes too long for the sample to exit the liner.
- Must be low enough to maintain sensitivity. If the split flow is too high, most of the sample will be vented to the atmosphere instead of entering the column. This can be useful when injecting concentrated samples, but small peaks in dilute samples could disappear altogether.
- Typical split flows are between 25 and 200 ml/ min.
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