Effect of Split Flow
These two chromatograms emphasize the importance of adequate split flow. In the top chromatogram, the split ratio is 50:1 but the analysis has been carried out on a 0.1 mm ID column. The flow through this column at 33.2 psi is only 0.22 ml/min (27 cm/sec). This would appear to be optimum conditions but the split flow is only 10.5 ml/min. Oven temp is isothermal at 150°C.
The second chromatogram shows analysis using the same conditions but the split flow has been increased to 50 ml/min (ratio = 250:1). Notice the dramatic improvement in the chromatography.
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