Problem Area 2

Problem Area 2 - The Column

• Do not heat until purged with oxygen- free gas (10 volumes)
• Always note injector and detector ends
• Always cut end of column after passing through ferrule
• Consider column dimensions and phase
• Consider the use of pre- column
• Set correct flow rate using linear carrier gas velocity
• Do not overheat column unnecessarily
• Use good quality ferrules and change as needed
• Support the column cage in the oven

Things that can deteriorate your column

• Very large volume injections
• Dirty samples
• Very acidic / basic samples
• Poor gas management
• Overheating

Restoring your Column

• Remove 50 cm to one metre from the injector end of the column
• Turn the column around and re-condition it
• Wash the column

Chromatograms >>