Problem Area 1 - The Injector- Do not heat until purged with oxygen- free gas (10 volumes)
- Always note injector and detector ends
- Always cut end of column after passing through ferrule
- Consider column dimensions and phase
- Consider the use of pre- column
- Set correct flow rate using linear carrier gas velocity
- Do not overheat column unnecessarily
- Use good quality ferrules and change as needed
- Support the column cage in the oven
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Carrier Gas - always use high quality gases
- use and regularly change gas filters
- use good quality cleaned tubing
| Septa- choose high quality low bleed septa
- change septa regularly
- consider tip style of syringe needle
| Other items to consider- column connection
- injection technique
- injection temperature
- solvent used
- split flow
- splitless time
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Syringe - use the correct one for the injector
- clean the syringe regularly
- never inject less than 20% full scale
| Injection liner- consider liner design
- change liner regularly
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