Syringes

I am using a syringe pump and need to calculate the pumping speed. What is the ID of my syringe barrel?
 
 Syringe Volume   such as Part Number  Internal Diameter (mm) 
 5µL  001310 (Removable) 

0.343 

 10µL  002310 (Removable) 

0.485 

 10µL  002312 (Removable) 

0.485 

 25µL  003312 (Removable) 

0.728 

 50µL  004312 (Removable) 

1.03 

 100µL  005312 (Removable) 

1.457 

 250µL  006312 (Removable) 

2.303

 500µL  007312 (Removable) 

3.257 

 1mL  008105 (Removable) 

4.606 

 1mL  008025 (Fixed)

4.606 

 2.5mL  008505 (Removable) 

7.284 

 2.5mL  008425 (Fixed) 

7.284 

 5mL  008762 (Fixed) 

10.301 

 10mL  008962 (Fixed) 

14.567 

 25mL  009463 (Fixed)

23.0 

 50mL  009660 (Fixed)

27.5 

 100mL  009760 (Fixed) 

35.0 

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Can I change the needle on a calibrated syringe and maintain the calibration?

With the exception of the 0.5 μL, 1 μL and 5 μL NanoVolume syringes, SGE syringes are calibrated using the internal dimension of the barrel, rather than measuring the volume that the syringe delivers. SGE syringes are therefore calibrated to contain, not calibrated to deliver.

Changing the needle won't change the volume contained in the syringe barrel, however it may change the amount delivered. To determine any effect of a needle change on the delivered volume, you should measure the delivered volume gravimetrically, or with a spectrophotometer.

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Can I check the dispensed volume of a syringe using a photometer?

The photometric method can be used as an alternative to gravimetric calibration of volume measuring devices (above).

A solution (usually a dye) of known absorbance at a particular wavelength is diluted to a known volume using the syringe to be calibrated. The theoretical dilution value (ratio of syringe volume to final volume) can then be checked against the actual dilution value (ratio of absorbance of initial solution to absorbance of diluted solution).

This method is limited by the absorbance range and accuracy of the photometer and the accuracy to which the volume of diluent can be measured.

Care must be taken with dye solutions, as they may stain the glass barrel of the syringe.

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What cleaning methods and maintenance procedures should be followed to ensure the calibration certificate remains valid? Do you recommend a special cleaning method, or a temperature at which these syringes should be kept? What should be avoided?

Calibrated syringes do not require different storage, cleaning, or handling to normal syringes. Anything that affects the accuracy and reproducibility of a normal syringe will also affect a calibrated one. The only particular thing to avoid would be altering the setting of a repeating adaptor, where the calibrated syringe has one.

A calibration certificate simply states that a syringe was made with certain internal dimensions and was delivered to the customer with those specifications.

Good laboratory practice dictates that wherever possible syringes should be used at a constant (room) temperature for maximum accuracy & reproducibility.

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What is the easiest way to check the dispensed volume of a syringe?

This is done by accurately weighing the amount of pure water that the syringe delivers (and recording the results a number of times).

You will need an analytical, or semi-micro, balance in a temperature controlled environment, free from vibration. Draw up the water and dispense it exactly the same way a number of times.

Wipe the outside of the needle so that no water adheres to it, taking care not to draw any water out of the needle itself.

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