ProteCol™ HQ for Proteins

ProteCol™-G C8 HQ1003 – The protein separation column


Since a 300Å pore size is not ideal for separating larger proteins, ProteCol™-G C8 HQ1003 column therefore uses a 1000Å pore size packing material.


Most proteins have a very hydrophobic core and they tend to unfold when coming in contact with a reversed phase surface. This can lead to very strong binding and sample loss. A C8 stationary phase gives much higher recoveries.


The example is the separation of the AOHUPO Membrane Protein Initiative mouse liver membrane proteins
standard.

 

ProteCol™ HQ1003 has a 0.7% carbon load, and can be used in a pH range of 1.0 – 8.0

 

Part no. Particle
Size 		ID
(mm) 		Length Pore
Size 		Column
Type 		Phase
Type 		Type
250170 3 2.1mm 150 1000 Glass Lined Tubing C8 HQ Analytical Column
250172 3 2.1mm 100 1000 Glass Lined Tubing C8 HQ Analytical Column
250175 3 300µm 150 1000 PEEKsil C8 HQ Capillary Column
250177 3 300µm 100 1000 PEEKsil C8 HQ Capillary Column
250180 3 150µm 150 1000 PEEKsil C8 HQ Capillary Column
250182 3 150µm 100 1000 PEEKsil C8 HQ Capillary Column
Part no. Particle
Size 		ID
(mm) 		Length Pore
Size 		Phase
Type 		Type Pack
Size
250017 3 4mm 10 1000 C8 HQ Guard Column 3
250019 3 2.1mm 10 1000 C8 HQ Guard Column 3