ProteCol™-G C8 HQ1003 – The protein separation column
Since a 300Å pore size is not ideal for separating larger proteins, ProteCol™-G C8 HQ1003 column therefore uses a 1000Å pore size packing material.
Most proteins have a very hydrophobic core and they tend to unfold when coming in contact with a reversed phase surface. This can lead to very strong binding and sample loss. A C8 stationary phase gives much higher recoveries.
The example is the separation of the AOHUPO Membrane Protein Initiative mouse liver membrane proteins
standard.
