ProteCol™ HQ for Peptides

ProteCol™-G C18 HQ203 and HQ303 – Columns for peptides

When analyzing samples containing large molecules (peptides, proteins, polymers with MW>3000) the size of the molecule and the size of the pore structure play an important role in the quality of the separation.  As the analyte increases in size (relative to the pore size) the diffusion rate inside the pore becomes smaller and mass transfer in and out of the pore system becomes slow leading to band broadening.  Obviously, when the analyte size is equal to or bigger than the pore size there can be no pore diffusion.  A mathematical description of this relationship was published by Renkin (E.M. Renkin, J.Gen.Physio., 38 (1954) 225.) and helps to illustrate the phenomenon.

 

ProteCol™-G C18 HQ203 and HQ 303 are columns packed with stationary phases of 200Å and 300Å pores, respectively.  They both show reasonably fast diffusion rates for a typical peptide (MW~5000) or any other molecule of a similar size.

The difference between them is the specific surface area (surface area are per unit of weight) which in case of the 200Å pore size material is twice as high as for the 300Å pore size particles (200 and 100 m²/g respectively). The surface area is directly linked to the column capacity.  The figure shows that in terms of resolution and peak capacity both columns provide very similar results.

 

 

ProteCol™ HQ203 has a 12.6% carbon load, and ProteCol™ HQ303 has a 7.4% carbon load.

Both can be used in a pH range of 1.0 – 8.0

 

Part no. Particle
Size (µm)		 ID Length
(mm)		 Pore
Size (Å)		 Column Type Phase
Type		 Description
2501205 4.6mm 250 300 Glass Lined Tubing C18 HQ Analytical Column
2501225 4.6mm 150 300 Glass Lined Tubing C18 HQ Analytical Column
2501255 2.1mm 250 300 Glass Lined Tubing C18 HQ Analytical Column
2501275 2.1mm 150 300 Glass Lined Tubing C18 HQ Analytical Column
2501303 2.1mm 150 300 Glass Lined Tubing C18 HQ Analytical Column
2501323 2.1mm 100 300 Glass Lined Tubing C18 HQ Analytical Column
2501353 300µm 150 300 PEEKsil C18 HQ Capillary Column
2501373 300µm 100 300 PEEKsil C18 HQ Capillary Column
2501403 150µm 150 300 PEEKsil C18 HQ Capillary Column
2501423 150µm 100 300 PEEKsil C18 HQ Capillary Column
2501503 2.1mm 150 200 Glass Lined Tubing C18 HQ Analytical Column
2501523 2.1mm 100 200 Glass Lined Tubing C18 HQ Analytical Column
2501553 300µm 150 200 PEEKsil C18 HQ Capillary Column
2501573 300µm 100 200 PEEKsil C18 HQ Capillary Column
2501603 150µm 150 200 PEEKsil C18 HQ Capillary Column
2501623 150µm 100 200 PEEKsil C18 HQ Capillary Column
Part no. Particle
Size (µm)		 ID Length
(mm)		 Pore
Size (Å)		 Phase
Type		 Description Pack
Size
2500213 2.1mm 10 200 C18 HQ Guard Column 3
2500233 4mm 10 200 C18 HQ Guard Column 3
2500255 2.1mm 10 300 C18 HQ Guard Column 3
2500275 4mm 10 300 C18 HQ Guard Column 3
2500293 2.1mm 10 300 C18 HQ Guard Column 3
2500313 4mm 10 300 C18 HQ Guard Column 3