ProteCol™ HQ for Peptides

ProteCol™-G C18 HQ203 and HQ303 – Columns for peptides

When analyzing samples containing large molecules (peptides, proteins, polymers with MW>3000) the size of the molecule and the size of the pore structure play an important role in the quality of the separation.  As the analyte increases in size (relative to the pore size) the diffusion rate inside the pore becomes smaller and mass transfer in and out of the pore system becomes slow leading to band broadening.  Obviously, when the analyte size is equal to or bigger than the pore size there can be no pore diffusion.  A mathematical description of this relationship was published by Renkin (E.M. Renkin, J.Gen.Physio., 38 (1954) 225.) and helps to illustrate the phenomenon.

 

ProteCol™-G C18 HQ203 and HQ 303 are columns packed with stationary phases of 200Å and 300Å pores, respectively.  They both show reasonably fast diffusion rates for a typical peptide (MW~5000) or any other molecule of a similar size.

The difference between them is the specific surface area (surface area are per unit of weight) which in case of the 200Å pore size material is twice as high as for the 300Å pore size particles (200 and 100 m²/g respectively). The surface area is directly linked to the column capacity.  The figure shows that in terms of resolution and peak capacity both columns provide very similar results.

 

 

ProteCol™ HQ203 has a 12.6% carbon load, and ProteCol™ HQ303 has a 7.4% carbon load.

Both can be used in a pH range of 1.0 – 8.0

 

Part no. Particle
Size 		ID
(mm) 		Length Pore
Size 		Column
Type 		Phase
Type 		Type
250120 5 4.6mm 250 300 Glass Lined Tubing C18 HQ Analytical Column
250122 5 4.6mm 150 300 Glass Lined Tubing C18 HQ Analytical Column
250125 5 2.1mm 250 300 Glass Lined Tubing C18 HQ Analytical Column
250127 5 2.1mm 150 300 Glass Lined Tubing C18 HQ Analytical Column
250130 3 2.1mm 150 300 Glass Lined Tubing C18 HQ Analytical Column
250132 3 2.1mm 100 300 Glass Lined Tubing C18 HQ Analytical Column
250135 3 300µm 150 300 PEEKsil C18 HQ Capillary Column
250137 3 300µm 100 300 PEEKsil C18 HQ Capillary Column
250140 3 150µm 150 300 PEEKsil C18 HQ Capillary Column
250142 3 150µm 100 300 PEEKsil C18 HQ Capillary Column
250150 3 2.1mm 150 200 Glass Lined Tubing C18 HQ Analytical Column
250152 3 2.1mm 100 200 Glass Lined Tubing C18 HQ Analytical Column
250155 3 300µm 150 200 PEEKsil C18 HQ Capillary Column
250157 3 300µm 100 200 PEEKsil C18 HQ Capillary Column
250160 3 150µm 150 200 PEEKsil C18 HQ Capillary Column
250162 3 150µm 100 200 PEEKsil C18 HQ Capillary Column
Part no. Particle
Size 		ID
(mm) 		Length Pore
Size 		Phase
Type 		Type Pack
Size
250021 3 2.1mm 10 200 C18 HQ Guard Column 3
250023 3 4mm 10 200 C18 HQ Guard Column 3
250025 5 2.1mm 10 300 C18 HQ Guard Column 3
250027 5 4mm 10 300 C18 HQ Guard Column 3
250029 3 2.1mm 10 300 C18 HQ Guard Column 3
250031 3 4mm 10 300 C18 HQ Guard Column 3