The ProteCol™-P C18 HPH125
The ProteCol™-P C18 HPH125 column has a specially modified stationary phase which allows it to be used outside commonly recommended pH ranges. While the silica matrix material of any silica based HPLC column has a stability limit of ~pH8, the special C18 modification of the support in combination with the PEEK™-lined column hardware enables the column to be run at pH9 and above without deterioration of performance.
In tests on the effects of elevated pH on ProteCol™-P C18 HPH125 chromatographic performance, we have observed
that peak symmetry is the most sensitive parameter to measure column performance compared to the often quoted retention time or the k’-value.
The figure below illustrates that peak symmetry on the ProteCol™-P C18 HPH125 column remains unchanged at pH 9.15 and over 3000 column volumes at 40°C. When compared to monomeric silica, peak symmetry deteriorated at less than 1000 column volumes whereas the retention time remained unchanged (see inset).
